| Congresso Brasileiro de Microbiologia 2023 | Resumo: 988-2 | ||||
Resumo:Avian salmonellosis is a disease triggered by bactéria belonging to the Salmonella genus,
affeting birds such as chickens and turkeys. In addition to causing economic losses and
reduced productivity in the poultry industry, in more severe cases, it can lead to mortality.
Preventing the disease involves several practices, including the use of appropriate
antimicrobials. Due to concerns about public health and food safety, effective control of
salmonellosis is essential in the industry. In this contexto, the vast Brazilian biodiversity
becomes na alternative to traditional synthetic antimicrobial agents. Therefore, this study
aimed to evaluate the antimicrobial activity of the ethanol and ethyl acetate extracts from
the native species Myrcia palustris DC. against 5 serotypes of Salmonella: S. Enteritidis,
S. Heidelberg, S. Orion, S. Mbandaka, and S. Infantis. The obtain the extracts, the plant’s
leaves were dried at 40ºC and ground using a knife mill. Subsequently, they underwent
vacum filtration and Rotary evaporation to remove all of the solvente. The minimum
inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were
determined using the broth microdilution method with serial dilutions ranging from 200-
0.09 mg/mL of the extact. Gentamicin at a concetration of 200 mg/mL was used as a
positive control. Both the ethanol and ethyl acetate extracts showed antimicrobial activity
against the five tested serotypes. Regarding the ethanol extract, a MIC and MBC of 12.5
mg/mL were observed for S. Mbandaka and 6.25 mg/mL for S. Orion. For the serotypes
S. Heidelberg and S. Enteritidis, the MIC values were 6.25 mg/mL, while the MBCs were
12.5 mg/mL, respectively. In the case of S. Infantis, the MIC was 12.5 mg/mL, and the
MBC was 25 mg/mL. On the other hand, the ethyl acetate extract exhibited antimicrobial
activity at higher concentrations. For S. Infantis and S. Orion, the MICs were 50 mg/mL
and the MBS were 100 mg/mL. In the case of serotypes S. Heidelberg, the MIC was 50
mg/mL, while the MBC was 200 mg/mL. Finally, for serotypes S. Mbandaka, the MIC
and MBC values were 25 mg/mL and 50 mg/mL, respectively, whereas for S. Enteritidis,
these values were 100 mg/mL and 200 mg/mL, respectively. Based on these results, it is
possible to conclude that the etanol and ethyl acetate extracts exhibit antimicrobial
potential, making them viable alternatives in the poultry industry. This represents a
natural and less harmful approach compared to synthetic antimicrobial agentes.
Additionally, it is importante to emphasize the need for further research to validate their
biological properties, ensuring their effectiveness in Salmonella control. Palavras-chave: antimicrobian activity, Myrcia palustris, plant extracts, plant extracts Agência de fomento:Unioeste, PPRN, CNPq | |||||